瘤浸润淋巴细胞抑制小鼠肺腺癌转移的研究

Tumor infiltrating lymphocytes inhibit spontaneouslung metastasis of LA_(795) adenocarcinoma in mice

采用Ｔ＿（７３９）近交系小鼠进行实验，发现小鼠ＬＡ＿（７９５）肺腺癌实体瘤中的瘤浸润淋巴细胞（ＴＩＬ）体外经白细胞介素２（ＩＬ＿２）激活，具有明显的抗肿瘤作用。体外杀瘤活性用氚－胸腺嘧啶核苷（￣３Ｈ－ＴｄＲ）释放法测定表明：ＴＩＬ的杀瘤活性明显高于自然杀伤细胞（ＬＡＫ）。体内的抗肿瘤作用主要观察ＴＩＬ抑制自发性肺转移作用。实验１静脉注射或瘤周注射ＴＩＬ的自发性肺转移抑制率分别为７３．５％，７９．９％；实验２的结果与实验１相似，且ＴＩＬ治疗组的自发性肺转移抑制作用强于ＬＡＫ细胞治疗组３倍。

umor InfiItrating Ly mphocytes(TILs)isolatedfrom LA_(795) lung adenocarcinoma in mice have a remarkable antitumor activity,by using T_(739) syngeneic mice.TILs cytolytic activity in vitro is higher than that ofLAK cells. TILs significantly inhibit spontaneous lungmetastasis in vivo. The results in experiment I showedthe following:TILs injection in tail vein,the inhibito-ry rate is 73.3%; TILs injection at tumor, 79.9%.The results in experiment II are similar to these in ex-periment I,and TILs antitumor activity in vivo is asthree times as that of LAK cells.