高效毛细管电泳法同时测定血浆中头孢羟氨苄和甲氧苄啶的含量

Determination of cefadroxil and trimethoprim in plasma by high performance capillary electrophorphoresis

采用毛细管区带电泳法(CZE)测定血浆中头孢羟氨苄、甲氧苄啶的含量.以20mmol/L硼砂(pH7.1)为背景电解质,35cm×75μm(i.d)未涂层毛细管为色谱柱,于267nm处检测,操作温度25℃,分离电压为20kV,采用峰面积外标法定量.头孢羟氨苄和甲氧苄啶的线性范围分别为0.4～4.0和0.25～4.0μg/ml,检出限分别为0.1和0.18μg/ml(S/N=3).该方法测得头孢羟氨苄和甲氧苄啶的在低、中、高浓度下的回收率均在95%以上,迁移时间的RSD分别为0.43%、1.44%;峰面积的RSD分别为3.34%、4.0%,日内、日间精密度均符合方法学要求.该方法用量少,简便,快速,准确,适合于血药浓度的监测.

A new capillary zone electrophoretic method was established for the rapid determination of cefadroxil and trimethoprim in plasma. The analysis was performed in an uncoated fused silica capillary (35cm×37.5μm), and a buffer solution of 20 mmol/L disodium tetraborate (pH7.1). The applied voltage was 20kV and UV detection wavelength was 267nm and the external calibration curve of peak area was selected. The (linear) ranges of cefadroxil and trimethoprim were 0.4～4.0 μg/ml and 0.25～4.0 μg/ml, respectively. The limit of detection for the former was 0.1 μg/ml and the latter was 0.18 μg/ml (S/N=3). The recoveries (obtained) from plasma for the two agents were more than 95%. The intra-day and inter-day precision assessed at three different concentration levels were satisfactory. The relative standard deviation (RSD) value for migration time of cefadroxil and trimethoprim were 0.43% and 1.44% respectively; the RSD value for peak area was 3.34% and 4.0%. The method is simple, rapid and accurate with less solvent consumption, and it can be (applied) to the identification and determination of the cefadroxil and trimethoprim in drugs monitoring.