TRAILmRNA与胃癌凋亡关系的研究

Relationship Between TRAILmRNA and Gastric Carcinoma Apoptosis

目的检测肿瘤坏死因子相关凋亡诱导配体(TRAIL)mRNA在胃癌中的表达及其与凋亡的关系。方法利用原位杂交技术检测67例胃腺癌及对应的正常胃黏膜中TRAILmRNA的表达,同时利用原位末端标记技术(TUNEL)检测癌组织中的凋亡指数(AI),并分析它们之间的关系。结果①TRAILmRNA在正常胃黏膜中阳性表达率为95.52%,高于癌组织中的阳性表达率52.24%(P<0.05);②胃癌组织中细胞凋亡指数为1.85±0.66;③TRAILmRNA阳性组与阴性组中凋亡指数分别为3.06±0.45、1.21±0.13,两组间存在着显著性差异(P<0.05);④TRAILmRNA与胃癌组织中的患者的年龄、性别、肿瘤直径、部位、大体分型、浸润深度及有无淋巴结转移无明显的关系(P>0.05);与肿瘤的分化程度有关,分化程度越低,TRAIL的表达率越低(P<0.05)。结论TRAIL可能在胃癌凋亡过程中起重要作用。

Objective To detect the expression of TNF related apoptosis inducing ligand (TRAIL)mRNA in gastric carcinoma and the relationship of apoptosis.Methods Using in situ hybrization technology to detect the expression of TRAIL in 67 cases of gastric carcinoma and normal mucosa,at the same time to detect the apoptosis index(AI)in cancer tissues using TUNEL technology.Results ①The positive rate of TRAIL in normal mucosa(95.52%) was higher than it in cancer tissues (52.24%)(P<0.05).②The AI was 1.85±0.66 in gastric carcinoma tissues.③The AI between the TRAILmRNA positive group was (3.06±0.45) and negtive group was (1.21±0.13).The difference was clear(P<0.05).④There was no obvious relationship among TRAIL mRNA and tumors diameter,site,gross classification,invasion depth,lymphnodes metastasis and the patients' age and sex(P>0.05),and there was no significant relationship with tumour differentiation. The expression of TRAIL mRNA was lower accompany with the differentiation degree (P<0.05).Conclusion TRAIL may play an important role in the gastric cancer apoptosis procession.