输血对环磷酰胺合并全淋巴照射的骨髓移植大鼠存活的影响

Effects of blood transfusion and cyclophosphamide before totallymphoid irradiation on survival of rats with bone marrow transplantation

作者以环磷酰胺合并６０Ｃｏγ射线一次１０Ｇｙ全淋巴照射（致死剂量）后进行骨髓移植，探讨了移植前后不同时间和不同供体输血对大鼠存活的影响．对照组和单给输血组于照后４天～１２天全部死亡；骨髓移植后１天同供体输血组６０天存活率与单给骨髓移植组相同（１０％），移植前１天同供体和非同供体输血的两组分别为２０％和４０％（Ｐ＜０．０５），移植前３天同供体输血组发生过敏加速死亡（全部死于４天～１０天）．当分次使用环磷酰胺时，移植前１天同供体输血组６０天存活率提高至８０％（Ｐ＜０．０１）．结果表明输血对大鼠骨髓移植疗效的影响与输血时间有关．

The effects of blood transfusion atvarious intervals before and afterbone marrow transplantation (BMT)and with different donors on thesurvival of rats with BMT were investigated. Cyclophosphamide wasadministered before total lymphoidirradiation (TLI) with 10Gy γ-raysfrom a 60Co source. All the ratsin control groups and in the groupwith blood transfusion alone diedwithin 4-12 days after TLI. The 60day survival rate after TLI in thegroup of donor-specific blood transfusion given one day after BMT wasnot significantly different fronthat in the group with BMT alone(the 60-day survival rate was10%). The survival rates in thegroups with transfusion of bothdonorspecific and nonspecific blood oneday before BMT were 20% and 40%(P<0. 05 ) respectively.All the rategiven blood transfusion three daysbefore BMT died within 4-10 daysafter TLI. The survival rate in thegroup with both donor-specific bloodtransfusion and cyclophosphamidegiven in divided does one day beforeBMT increased to 80% (P<0 .01).The results show that the tehrapeutic effect of bolld transfusion onrats with BMT is related to thetime of blood transfusion.