脐血淋巴因子激活杀伤细胞的实验研究

Study on lymphokine-activated killer cell in-duced from cord blood

本研究用脐血淋巴细胞诱导产生淋巴因子激活杀伤（ＬＡＫ）细胞，并且比较脐血ＬＡＫ细胞与正常成人和妇科肿瘤患者外周血ＬＡＫ细胞的杀伤活性；利用单克隆抗体研究脐血ＬＡＫ细胞表面抗原变化以及在电镜下观察ＬＡＫ细胞杀伤靶细胞时的形态学变化。研究结果表明：（１）脐血可诱导出ＬＡＫ细胞，脐血ＬＡＫ细胞能明显杀伤传代的肿瘤细胞系，包括对自然杀伤细胞（ＮＫ）敏感的Ｋ＿（５６２）细胞和对ＮＫ抵抗的Ｒａｊｉ细胞；（２）脐血ＬＡＫ细胞活性显著高于妇科肿瘤患者外周血ＬＡＫ细胞活性，但与成人外周血ＬＡＫ细胞无明显差异；（３）脐血ＬＡＫ细胞的ＯＫＴ抗原为Ｔ３￣＋和Ｔ８￣＋，与其他来源的人ＬＡＫ细胞相似；（４）脐血ＬＡＫ细胞与Ｒａｊｉ细胞共育一定时间后，在光镜下可见两者形成不典型的玫瑰花状结合物，在透射电镜下，脐血ＬＡＫ细胞伸出突起，主动与靶细胞结合。

In this article. the studies on the method of in-ducing lymphokine-activated killer (LAK) cells fromcord blood lymphocytes, the killing ability of LAKcells to tumor subculture cells K_562 and Raji, thecomparison of the killing activity to tumor subculturecells among LAK cells from cord blood lymphocytes.adult′s Peripheral blood lymphocytes (PBL) andgynecologic cancer patient′s PBL was presented. Thechanges of the OKT antigens on the surface of cordblood LAK cells tested with monoclonal antibodieswas discribed. The morphologic changes resulted fromthe killing activity of LAK cells to target cells were ob-served by microscopy and electron-microscopy after adefinite time co-incubation of the LAK cells and Rajicells.The results showed that:(1) LAK cells could beinduced from cord blood and the induced LAK cellscould remarkably kill the tumor subculture cell linesincluding nature killer (NK)-sensitive K_562 cells aswell as NK-insensitive Raji cells; (2) The activity ofcord blood LAK cells is higher than that of the LAKcells from gynecologic cancer patient's PBL, But nosignificant difference with that of the LAK cells fromnormal adult's PBL; t3) T+3. T+s, the OKT antigens ofcord blood LAK cells are just the same as humanLAK cells from other sources; f4) After a definite timeco-incubation, the cord blood LAK cells and Rajicells could form atypical rosette -circles under lightmicroscopy. In the field of transmissionelectron-microscopy, the cord blood LAK cells had acertain active mobility. Raji cells, when incubated withthe cord LAK cells for 4 hours, would becomeapoptosis. It indicated that the LAK cells showedsome properties during their last period of killing thetarget cells similar to that of NK cells and cytotoxic Tlymphocyte fCTL).