葡萄胎滋养细胞DNA及RNA含量的定量分析及临床意义

Quantitative analysis of the cellular DNA andRNA contents of hydatidiform mole and itsclinical significance

应用流式细胞分析技术对６９例良性葡萄胎（良葡组）和８３例恶性葡萄胎（恶葡组）组织的滋养细胞ＤＮＡ和ＲＮＡ含量进行定量分析。结果：ＤＮＡ指标对良性葡萄胎诊断准确率为６６．７％，ＲＮＡ指标对良性葡萄胎诊断准确率为８２．６％；在恶葡组ＤＮＡ指标预测恶变的准确率为６８．７％，ＲＮＡ指标为８１．９％。ＲＮＡ指标的准确率显著高于ＤＮＡ指标（Ｐ＜０．０５）。两指标同时预测恶变的准确率为９４．０％。提示：ＤＮＡ及ＲＮＡ含量可作为判断葡萄胎性质及预测恶变的客观指标，ＲＮＡ指标较ＤＮＡ指标更敏感，两指标同时应用优于任一单项指标。

he cellular DNA and RNA contents of 152 pa-tients with hydatidiform moles ( 69 benign and 83 malig-nant) were analysed by flow cytometry (FCM) , andstrict follow-up was implementcd. The ctiioriouic villi oftwelve cases in early pregnancy were used as normalcontrol and another 12 cases of choriocarcinoma as posi-tive control. Retrospective as well as prospective studieswere conducted. The results showed that the accuracyrates of diagnosis of DNA aneuploid and RNA were re-spectively 66. 7% and 82. 6% in benign moles, 68. 7%and 81. 9% in invasive moles. Statistically the RNA pa-rameter was significantly more sensitive the DNA pa-rameter (p<0. 05 ). Analysis with both RNA andDNA parameters in predicting malignancy , the accuracyrate was 94. 0%. Our study suggests that both the ane-uploidy DNA and RNA contents may be important indi-cators for forecasting mahgnant change. The RNA pa-rameter is more sensitive than the DNA parameter, andthe use of both parameters is more accurate than eitherone alone. In addition, the concept of“standtard DNAindex”is raised.