应用化学发光酶免疫法测定妇科肿瘤组织雌激素受体

A controlled study of chemiluminescent enzymeimmunoassay for the measurement of estrogenreceptor in tissue of gynecological neoplasms

为了寻找一种理想的性激素受体的检测方法，本研究首先应用化学发光酶免疫法（ＣＬＥＩＡ）测定３４例妇科肿瘤（子宫内膜癌１５例、卵巢肿瘤１５例及其他肿瘤４例）组织的雌激素受体（ＥＲ），并与葡聚糖包裹活性碳法（ＤＣＣ）及酶免疫测定法（ＥＩＡ）进行对照。结果：（１）ＣＬＥＩＡ标准曲线显示发光强度和ＥＲ浓度线性关系良好（ｒ＝０．９７８６）。（２）ＣＬＥＩＡ测得ＥＲ值与ＤＣＣ或ＥＩＡ所测ＥＲ值的相关性极显著（Ｐ＜０．００１），相符率＞９０％。（３）三种方法测得的ＥＲ阳性率，差异无显著性，而ＥＲ均值以ＣＬＥＩＡ为最高。结论：ＣＬＥＩＡ测定ＥＲ的敏感性较另两法为高。

n this study, chemiluminescent enzyme im-munoassay (CLEIA) has been developed for the mea-surement of estrogen receptor (ER) in 34 gynecologicaloncological tissues from 15 cases of endometrial carci-noma, 15 cases of ovarian tumors and 4 cases of othergynecological tumors. The accuracy , specificity and sen-sitivity of CLEIA for ER were compared with that ofdextrancoated charcoal (DCC) and enzyme immunoas-say (EIA).Results: (1) the standard curve of ER-CLEIAshowed a significant llnear correlation between the lumi-nosity and ER concentration (r=0. 9786 , P<0. 001);( 2) The results of CLEIA and that of the other twomethods were compared; the correlation coefficientswere>0. 8 and their corresponding rates were>90%;; (3)the positive rate of ER determind by these threemethods showed no significant differences, however,the mean value of ER-CLEIA was the highest amongthem.It is concluded that the CLEIA has a higher sensi-tivity than that of the other two methods for the mea-surement of ER.