应用肿瘤引流区淋巴结细胞诱导淋巴因子激活杀伤细胞的特性

The characteristic of lymphokine-activatedkiller cells induced from tumor draininglyumph-nede cell

对７例妇科恶性肿瘤患者肿瘤引流区域淋巴结的淋巴细胞，在体外进行短期淋巴因子激活杀伤（ＬＡＫ）细胞的诱导培养。结果表明，ＬＡＫ细胞对肺癌细胞系、卵巢癌ＣＡＯＶ＿３细胞系及新分离的卵巢癌细胞杀伤能力不同，且效应细胞与靶细胞比例在４０：１以上时，ＬＡＫ细胞活性维持在一定水平；该ＬＡＫ细胞对同种分离卵巢癌细胞杀伤能力更强。白细胞介素。２（ＩＬ－２）和ＯＫＴ＿３联合应用诱导ＬＡＫ细胞，随着培养时间的延长，其杀伤靶细胞的能力强于单独使用ＩＬ－２诱导的ＬＡＫ细胞。肿瘤引流区域淋巴结经分离可获得大量的淋巴细胞。

short time lymphocyte culture for lymphokineactivated killer (LAK) cells was presented , the lympho-cytes being collected from the tumor draining regionallymph nixles in seven patients with gynecologic malig-nant tumors. The results showed different cytotoxic ac-tivities of LAK cells against Lewis pulmonary cacercells ,CAOV_3 cells and the new separated ovarian cacercells. And the cytotoxic activity of the LAK cells in-creased with the rise of the effecter cell-target cell ratioand maintained at a certain level when the rise of thelatter's increase reached a certain extent. The cytotoxicactivity of the LAK cells against autologous tumor cells(as from ovarian cancer) were shown to be muchstronger than against the others. As the culture dayslengthened , the cytotoxicities of the LAK cells from interleukinZIOKT 3 culture bacame stronger than frominterloukin-2 alone. Large quantities of lymphocytescould be collected from tumordraining lymphe nixles.This is a preliminary investigation on the inducing ofLAK cells from lymphocytes of the draining regionallymph nedes of malignant gynecologic tumors. Thequantitative increase of LAK cells and the effect of theircytotoxic activities are to be further explored.