摘要
目的:研究NY-ESO-1基因在胃癌中的表达,以便了解为胃癌患者是否可以使用NY-ESO-1抗原为基础的疫苗治疗。方法:采用RT-PCR方法检测了NY-ESO-1基因在60例胃癌组织和相应的癌旁正常组织中的表达,采用分子克隆的方法从胃癌组织中克隆了NY-ESO-1cDNA。结果:NY-ESO-1基因在胃癌组织中的表达率为55%(33/60),在相应的癌旁正常组织中的未见表达,克隆的NY-ESO-1cDNA序列与GenBank报道一致。结论:NY-ESO-1基因在胃癌中的高表达率,NY-ESO-1抗原可以被具有HLA-1类分子限制性CTL识别。
Objective To study the expression of NY-ESO-1 in gastric carcinoma, and so as to know if NY-ESO-1 can be as a kind of fundamental gene vaccine in the treatment of gastric carcinoma. Method Reverse transcriptase and polymerase chain reaction amplification techniques were utilized to assay 60 gastric carcinoma tissues and the matched adjacent normal gastric tissues for expression of NY-ESO-1 gene. And the cDNA sequence of NY-ESO-1 was cloned from gastric carcinoma tissues by molecular cloning techniques. Results Out of 60 gastric carcinoma tissues, 55% expressed NY-ESO-1 gene while no expression in the matched adjacent normal gastric carcinoma tissues. The sequence of NY-ESO-1 cloned was identical to the sequence of that from GenBank. Conclusions NY-ESO-1 gene is expression highly in gastric carcinoma tissues and NY-ESO-1 antigen can be recognized by cytolytic T lymphocytes when presented by HLA-class-1 molecular.
出处
《吉林医学》
CAS
2005年第6期576-577,共2页
Jilin Medical Journal
基金
深圳市科技和信息局资助项目(200405186)
