荧光相关光谱及其在单分子检测中的应用进展

Advances in Fluorescence Correlation Spectroscopy and Its Applications in Single Molecule Detection

单分子检测在生命科学、化学、物理学等领域具有重要的意义。荧光相关光谱是单分子检测的新技术,在生命科学领域有巨大的应用潜力。综述了荧光相关光谱单分子检测的原理、实验技术以及在生物分子相互作用、活细胞、核酸、疾病诊断、高通量筛选以及与毛细管电泳联用等领域的研究,并展望了其发展前景。

Single-molecule detection is playing great role in the field of life science, chemistry and physics. Fluorescence correlation spectroscopy (FCS) is an attractive alternative technique for detection of single molecule, especially for single bio-molecule. The principle, methodology of FCS and its key applications in interaction of bio-molecules, living cells, nucleic acids, diagnostics, high-throughput screening and coupling with capillary electrophoresis were reviewed. The prospects of FCS in single molecule detection were also discussed.