摘要
单分子检测在生命科学、化学、物理学等领域具有重要的意义。荧光相关光谱是单分子检测的新技术,在生命科学领域有巨大的应用潜力。综述了荧光相关光谱单分子检测的原理、实验技术以及在生物分子相互作用、活细胞、核酸、疾病诊断、高通量筛选以及与毛细管电泳联用等领域的研究,并展望了其发展前景。
Single-molecule detection is playing great role in the field of life science, chemistry and physics. Fluorescence correlation spectroscopy (FCS) is an attractive alternative technique for detection of single molecule, especially for single bio-molecule. The principle, methodology of FCS and its key applications in interaction of bio-molecules, living cells, nucleic acids, diagnostics, high-throughput screening and coupling with capillary electrophoresis were reviewed. The prospects of FCS in single molecule detection were also discussed.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第6期875-880,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20271033
20335020)
上海市自然科学基金(No.02ZA14057)
中国博士后科学基金(No.200303264)资助项目