IFN-γ基因转导A549细胞对异种小鼠S_(180)抑瘤作用

Inhibitive Effect of IFN-γ Gene Transduced A549 Cell on Xenogeneic S180-bearing Mice

[目的]探讨γ鄄干扰素(IFN鄄γ)转基因疫苗对异种小鼠S180肿瘤的抑瘤作用及其影响因素。[方法]将IFN鄄γ基因修饰的转化细胞株,经γ鄄60Co照射后制成转基因疫苗,测定其MHC鄄Ⅰ、Ⅱ分子的表达,以及60Co照射与冻融等因素对其影响。在添加肿瘤细胞裂解物和GM鄄CSF、IL鄄2及变换注射部位等,观察对异种荷瘤S180小鼠的抑瘤作用。[结果]经IFN鄄γ基因修饰后,其细胞表达MHC鄄Ⅰ、Ⅱ分子明显提高,该两分子的上调并不受照射及冻融等因素的影响。变换注射部位及用IL鄄2取代转基因疫苗作预防性注射后依然有抑瘤作用。[结论]经IFN鄄γ基因修饰的A549细胞疫苗其MHC鄄Ⅰ、Ⅱ分子的上调并不受照射、冻融等因素及变换注射部位的影响,仍可对异种动物肿瘤产生较强的免疫抑瘤作用。

Purpose] To investigate the inhibitive effect and related factors of the γ-interferon(IFN-γ) transgenic vaccine on xenogeneic S180-bearing mice. [Methods] IFN-γ gene transduced vaccine was obtained from IFN-γ transformation cell line after 60Co radiation. MHC-Ⅰ?Ⅱ molecule expressing levels were detected and their changes were evaluated by means of the radiation and freezing. Inhibition effect on the xenogeneic S180-bearing mice was evaluated after changing the injection site and adding GM-CSF, IL-2 and tumor cell lysates. [Results] The cell line could express high MHC-Ⅰ?Ⅱ levels after being transduced by IFN-γ gene which were not affected under 60Co radiation and freezing condition. The inhibition affects were not effected after changing the injection site and using IL-2 as a replacing transgenic vaccine. [Conclusion] The results suggest that the IFN-γ transgenic A549 cell vaccine under different condition such as: radiation and freezing, or change injecting site, or using IL-2 to preplace the transgenic vaccine could still have a strong inhibitive effect on the xenogeneic mouse tumor.