摘要
选用无毒、无免疫原性的高分子化合物聚乙二醇(PEG)为修饰剂,以活性酯法制备出PEG-重组人超氧化物歧化酶(PEG-rhSOD)共价结合物。修饰酶的生物半衰期为15h,比修饰前延长了90倍,对酸、硷、热的耐受力明显高于修饰前。用照射小鼠活存率评价了PEG-rhSOD的辐射防护作用。小鼠受8.5Gvr射线照射前1h腹腔注射30万U/kgPEG-rhSOD,可提高活存率42%,与未修饰的rhSOD相比,活存率提高14%。
ecombinent humtan Cu/ZnSOD(rhCu/ZnSOD)was covalently
linked with activated polyethylene glycol(PEG),a kind of nontoxic,nonimmunogenic and
biocompatible polymer,The modified enzyme,PEG-rhCu/ZnSOD,has longer half-life(15h).Its
abilitie:to resist acid,alkali and heat were increased significantly.Mice given PEG-rhCu/ZnSOD
1h bcfore irradiation had higher survival rate than those not given or given rhCu/ZnSOD after
they were exposed to whole body Y-ray irradiation at dose of 8。5Gy。
关键词
辐射防护剂
超氧化物歧化酶
生物制品
研制
Radioprotecive agent Superoxide dismutase Geneticengineering Chemical modification