半无菌表面灭菌获得葡萄单芽茎段无菌材料的方法

A half - sterilized method acquiring sterilized stem - segments with single bud of grape

介绍了对当年生葡萄嫩枝利用半无菌灭菌方法结合75%酒精处理3-5s,无菌水洗一次,0.1%升汞3min,进行表面灭菌,无菌处理效果好,污染率低,且单芽萌发情况好。

The method of half-sterilized and 75% alcohol 3-5s, sterile water washing and 0. l%HgCl2 3min has a good sterilizing effect on grape. The rate of pollution is low and single bud grows very well.