Y迷宫训练对成年大鼠海马齿状回细胞的增殖作用

Y Maze Learning Increases Cell Proliferation in the Hippocampal Dentate Gyrus of Adult Rats

目的观察Y迷宫训练对成年大鼠海马齿状回(dentategyrus,DG)神经干细胞/前体细胞的增殖作用。方法通过Y迷宫训练,用5溴2脱氧尿嘧啶核苷(5bromo2deoxyuridine,BrdU)标记增殖细胞,用ABC免疫细胞化学方法观察成年大鼠海马齿状回细胞的增殖情况。结果Y迷宫训练组海马齿状回BrdU阳性细胞数明显多于对照组(P<0.05),且Y迷宫训练成绩的优劣与海马齿状回神经细胞增殖的多少存在相关性,学习成绩良好组齿状回BrdU阳性细胞数显著多于学习成绩低劣组(P<0.05)。结论Y迷宫训练可促进成年大鼠海马齿状回神经干细胞/前体细胞的增殖。

Objective To observe the effect of Y maze learning on neural stem cell/progenitor proliferation in the hippocampal dentate gyrus of adult rats. Methods The cell proliferation of dentate gyrus of adult rats was observed by using BrdU(5-bromo-2-deoxyuridine,BrdU) to mark cell proliferation and ABC(avidin-biotin-complex,ABC)immunocytochemical technique after Y maze learning.Results BrdU-positive cells of Y maze learning group were more than that in the control group (P<0.05).There was a correlation between the performances of Y maze learning and cell proliferation of dentate gyrus.BrdU-positive cells in rats with good performances in learning was higher than that in rats with bad performances(P<0.05). [WTHZ]Conclusion Y maze learning increases neural stem cell/progenitor proliferation in the hippocampal dentate gyrus of adult rats.