人脑胶质瘤细胞株SHG-44放射敏感性的测定方法探讨

The Discussion of Method for Survey the Radiosensitivity of Human Glioma Cell Line SHG-44

目的探讨MTT法、细胞计数Kit8(CCK8)法在进行人脑胶质瘤细胞株SHG44细胞放射敏感性测定时能否替代集落形成试验法。方法运用3种方法分别在0、1、2、3、4、6、8、10Gy8个不同剂量点照射后检测其存活分数,采用统计学检查分析3种方法所得存活分数的相关性。结果在一定照射剂量内(照射剂量≤3Gy时),MTT法、CCK8法与克隆形成法相关性较好,而超出该剂量范围时的相关性差。经直线回归分析,3种方法均不存在高度线性相关。CCK8和MTT法相比无更好的相关性。结论集落形成法仍然是测定放射敏感性的“金标准”,MTT法等其他方法可以提供参考,但无法替代集落形成法。

Objective To investigate if thiazolyl blue colorimetric assay(MTT) and cell counting kit-8(CCK-8) can replace clone forming assay for survey the radiosensitivity of SHG-44.Methods Three assays was applied to examine the growth inhibition of human glioma cell line SHG-44 in eight dose groups of 0,1,2,3,4,6,8 and 10 Gy,and statistical research was applied to analyze the correlation between survival fraction and various doses.Results Dose was associated with survival fraction in these three assays at some range of irradiation dosage(dose≤3 Gy).If out of the range, the relation is poor. CCK-8 has no rather superiority than MTT.Conclusion By now clone forming assay is still the “gold standard”. In some cases, MTT and other assays can give us some reference, but these assays still can not replace clone forming assay.