梓醇对缺血再灌注受损伤神经元保护作用的研究(英文)

Study on protective effect of catalpol against injury of ischemia-reprefusion

目的观察梓醇在沙土鼠短暂性脑缺血再灌注中的作用。方法健康沙土鼠,随机分为:假手术组,缺血组和梓醇治疗组。采用短暂性双侧颈总动脉夹闭制作脑缺血再灌注模型;利用电镜观察缺血后细胞形态变化情况;用流式细胞术检测缺血后凋亡细胞的数量;经TTC组织酶染法显示脑组织梗死区域后进行图像分析,计算缺血后脑组织梗死面积的百分数。结果与缺血组相比,梓醇治疗组海马CA1区锥体神经元细胞形态基本恢复正常;凋亡细胞数明显降低(P<0.05);梗死面积显著下降(P<0.05)。结论梓醇对缺血再灌注受损神经元有保护作用。

[Objective] To investigate the effect of catalpol in gerbils brain i schemia-reprefusion model. [Methods] Gerbils were randomly divided into three groups: sham, ischemia and catalpol. The ischemia-reprefusion model was develope d by shortly occluding common carotid arteries. After ischemia, the change situa tion of chromatin was observed by electron microscope. Cell number of apoptosis is detected with flow cytometry. The infarction fields of brain were displayed b y TTC dye. Image analysis was used to measure the percentage of the infarction a rea. [Results] Compared with the ischemia group, the morphomogy of neurons in hippocampal CA1 was normal in the group treated with catalpol. The cell number o f apoptosis markedly decreased (P<0.05). And the area of infarction was also sig nificantly reduced (P <0.05). [Conclusion] Catalpol was of the effect of neuro protection.