非甲-非戊型肝炎患者血清自身抗体的检测及分析

Clinical analysis of autoantibodies in non-A non-E hepatitis

目的探讨非甲-非戊型肝炎患者血清自身抗体的存在情况及临床意义。方法采用ELISA法筛选28例非甲-非戊型肝炎患者,用间接免疫荧光法和蛋白质印迹法分别检测所有研究对象的抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA-M2)、抗肝肾微粒体抗体(ALKM)、抗可溶性肝抗原抗体/抗肝胰抗体(ASLA/ALP)、抗肝细胞胞浆抗原I型抗体(LCIA)。结果11例非甲-非戊型肝炎患者检出至少一种以上自身抗体,自身抗体总检出率达39.3%。未检出抗SLA/ALP及抗LCIA。经完善相关检查并结合临床特点,最终有6例患者诊断为自身免疫性肝病(自身免疫性肝炎-I型3例、Ⅱ型1、原发性胆汁性肝硬化2例)。结论非甲-非戊型肝炎可存在自身免疫性肝病和其他类型肝炎病毒。在非甲-非戊型肝炎患者中常规检查抗肝抗原自身抗体有助于疾病的诊断和鉴别诊断。

[Objective] To investigate the clinical significance of autoantibodi es in non-A, non-E hepatitis. [Methods] 28 cases of non-A, non-E hepatitis pat ients were identified by enzyme linked immunosorbent assay (ELISA). ANA, ASMA, A MA-2, ALKM, ASLA/ALP, ALC I A were measured by using indirect immunofluorescent assay, western blotting. [Results] Autoantibodies including ANA, ASMA, AMA-2, ALKM were found in 11 cases of Non-A, Non-E Hepatitis and the positive rates of autoantibodies was 39.3%. we did not found the autoantibodies of SLA/ALP and ALC IA . Through relavant examination and clinical features, 6 patients were diagno sed autoimmune liver diseases (including 3 cases of AIH-I, one case of AIH-II, 2 cases of PBC). [Conclusion] Autoimmue liver disease and other hepatitis virus may be present in Non-A, Non-E Hepatitis. The detection of autoantibodies in No n-A, Non-E Hepatitis patients is helpful for diagnosis and identified diagnosis of diseases.