锂对原代培养的猪甲状腺细胞生长的影响

Effect of Lithium on the Growth of Cultured Pig Thyroid Cells

目的 研究锂在原代培养的猪甲状腺细胞生长调节中的作用。方法 选取新鲜猪甲状腺组织,采用细胞原代培养技术和非同位素标记的细胞增殖分析法(MTT) ,检测不同浓度的氯化锂(LiCl)对甲状腺细胞生长的影响。结果 在TSH存在及不存在条件下,LiCl均呈剂量依赖性的促进甲状腺细胞的生长。结论 一定浓度的LiCl能够促进甲状腺细胞增殖。

Objective To investigate the effect of Lithium on the growth of pig thyroid gland. Methods Fresh pig thyroid cells were cultured in a monolayer system.Non-radioactive Cell Proliferation Assay(MTT) was used to detect different concentration Lithium on the growth of pig thyroid cells. Results In the condition of non-TSH and TSH,Lithium both enhance the growth of pig thyroid cells in dose-dependent manner. Conclusions Lithium can accelerate the growth of pig thyroid cells.