同种异系抗原不同途径诱导大鼠免疫低反应性的研究

Study on Abrogation of the Capacity of Delayed-type Hypersensitivity Responses to Alloantigens and Suppression of Mixed Lymphocyte Reaction by the Different Approaches with allogeneic Cells in Rat

目的 比较同种异系抗原通过不同途径接种后机体对该抗原免疫反应状态的变化,探讨口服耐受的机制。方法 以SD大鼠为供者,Wistar大鼠为受者,将SD大鼠脾细胞,分别以口服、联合(口服+门静脉注射)两种途径接种于Wistar大鼠,测定供受体之间单向混合淋巴细胞培养(MLC)和受体对供体抗原的迟发性超敏反应(DTH)。结果 两种途径与对照组相比MLC (A)、DTH脚掌厚度增值均显著降低,其差异有显著性(P<0 .0 5 )。结论 两种途径均可诱导机体对该抗原的免疫低反应性。

Objective To compare the change of the level of immunoreactions to allogeneic cells after receiving inoculation by the different approaches with allogeneic cells in rats,and to study the mechanism of oral tolerance. Methods SD and Wistar rats were used as donors and recipients respectively,and splenocytes from SD were inoculated into Wistar by taking orally and injection via portal vein combined with taking orally.Mixed lymphocyte culture(MLC) of Wistar to SD rats alloantigens was investigated and anti-SD delayed-type hypersensitivity(DTH) responses assessed by directly challenging SD splenocytes. Results The two revulsive approaches significantly abolished the capability of the animals to develop MLC responses and DTH responses to SD alloantigens as induced by the subcutaneous immunization(P<0.05 ) . Conclusions The two revulsive approaches could induce low immunoreaction to alloantigens.