TH1反应在铜绿假单胞菌二次感染小鼠中作用的研究

The Role of TH1 Type Response in Re-infected by Pseudomonas Aeruginosa

目的 探讨TH1对铜绿假单胞菌(PA) 2次感染小鼠的保护作用。方法 将6 8只12周龄、雌性、BALB/C小鼠,随机分为3组。A组2 0只为1次感染组,B组2 8只为2次感染组,C组2 0只为非感染组。在首次用生物被膜PA接种小鼠肺部2周后进行第2次接种,建成小鼠PA 2次肺部感染模型,用A组和C组作对照组。用酶联免疫吸附法(ELISA)检测小鼠肺组织中白介素IL - 4 ,干扰素IFN -r、IL - 4 /IFN -r及IL - 12水平。结果 白介素IL - 4B组明显下降,与A组和C组间差别均有非常显著性意义(P <0 . 0 1) ,干扰素IFN -rB组明显升高,与C组比较差别有非常显著性意义(P <0 . 0 1) ,但B组与A组未见明显差别(P >0 .0 5 )。结论TH1主导的细胞因子反应在铜绿假单胞菌对2次感染小鼠起一定的保护作用。

Objective To study role of TH1 type response in re-infected by pseudomonas aeruginosa(PA).Methods 68 mice were divided into three groups at random.In Group B,mice were re-infected by PA.In Group A and Group C,mice were singly-infected by PA and no-infected by PA, were used as controls.To measure kinetics concentrations of IL-4,IL-12 and IFN-r in lung by ELISA. Results Resistance to re-infection was paralleled by a shift towards a TH1 dominated response and increased IL-12 production. Conclusions These results indicated a protective role for a TH1 dominated response, independent of antibody production, in chronic lung infection of pseudomonas aeruginosa in mice.