DNA斑点杂交检测大肠癌C－myc癌基因扩增的临床意义

THE RELATIONSHIP BETWEEN CLINICOPATHOLOGIC PARAMETERS AND C-myc AMPLIFICATION IN COLORECTAL CARCINOMA WITH DNA DOT-BLOT HYBRIDIZATION

应用ＤＮＡ斑点杂交技术对４４例大肠癌及相邻正常粘膜组织Ｃ－ｍｙｃ癌基因扩增进行分析，探讨Ｃ－ｍｙｃ扩增与大肠癌分化程度及Ｄｕｋｅｓ分期的关系．结果表明：大肠癌Ｃ－ｍｙｃ扩增率为６１．４％．并与分化程度密切相关，粘液腺癌和低分化腺癌的扩增率明显高于高分化腺癌．Ｃ－ｍｙｃ扩增可作为大肠癌的一项有价值的生物学标志．

Abstract An amplification of c-myc oncogene was investigated hybridizatially in 44 colorectal carcinoma tissues and their adjacent normal colon mucosa. The correlation of amplification with tumor grade of differentiation and Dukes stage was analysed. DNA dot-blot hybridization showed 2￣ 7 fold amplification of the c-myc gene in 61 .4% tumor, and statistical analysis revealed a significant correlation between c-myc and grade of tumors. An amplification ratio of c-myc was 84.6% in mucinous carcinoma, and 71 .4% in poorly differentiated tumors.