摘要
从人心肌组织中提纯并鉴定了心肌肌钙蛋白-1(CTnI),免疫新西兰纯系兔而获特异性抗血清,其亲和常数Ka值为4.1×1010L/mol.Iodogen法制125I-CTnI,比活度为3659GBq/g.建立了CTnI双抗体RIA,标准曲线ED50为6.4±0.34μg/L(x±s,n=8),灵敏度达1.5μg/L,工作范围为2-64μg/L,批内、批间CV分别为2.1%-5.7%(n=8)和5.4%-9.0%(n=4),方法回收率为90.1%-95.3%.与骨胳肌肌钙蛋白-I(STnI)未见交叉反应,测定87例正常人血清CTnI浓度为2.17±0.66μg/L(x±s),80例有关疾病患者CTnI血清浓度测定表明该方法对诊断心肌细胞损伤具有心肌特异性.并且CTnI于心肌细胞损伤后在血清中出现时间早,维持时间长,是一个理想的心肌细胞损伤标志物.
Abstract A double antibody radioimmunoassay (RIA) was developed to measure cardiac troponin-I (CTnI) in serum. CTnI from fresh human myocardium was purified and identified. Antisera against CTnI were raised in NZ rabbits with affinity constant (Ka) 4.1 × 10-10L/mol. Iodination of CTnI was carried out with iodogen. The specific activity of the product was 3659 GBq/g. The ED40 was 6.4±0.34μg/L (x±s, n=8) from standard curves. The sensitivity was 1.5μg/L and the assay covered the range 2 ̄ 64μg/L for CTnI. The intra-assay and inter-assay coefficient of variation (CV) were 2.1%  ̄5.7% (n=8) and 5.4% ̄9.9% (n=4) respectively. The assay recovery was 90.1% ̄95.3%.Non-immunochemical cross reactivity with skeletal troponin-I(STnI) was observed. The normal value was 2.17 ± 0.66μg/L (x ± s) in sera of 87 normal persons.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1994年第3期164-166,共3页
Chinese Journal of Nuclear Medicine