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RNAi技术的研究进展和发展前景 被引量：4

Research advances and further of RNAi technique
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摘要 生物体内导入双链RNA后会引起体内同源基因特异性的沉默,这种现象称为RNA干涉。文章主要介绍RNAi的特征、作用机制及应用等方面的情况。 RNA interference denoted the phenomenon that the homologous gene in vivo would be silenced when double strand RNA were transferred into organism. The characters, function mechanism and applications of RNAi were introduced in the paper.
作者 李成 张滨丽 李杰
机构地区 东北农业大学生命科学学院 黑龙江兽医卫生防疫站
出处 《东北农业大学学报》 CAS CSCD 2005年第3期365-368,共4页 Journal of Northeast Agricultural University
关键词 RNAI DSRNA 基因沉默 RNAi dsRNA gene silence
分类号 Q343.1 [生物学—遗传学]
  • 相关文献

参考文献21

  • 1Fire A, Xu S, Montgomery M K, et al. Potent and specific genetic interference by double-stranded RNA in caenorhabditis elegans [J]. Nature, 1998, 391(6669): 806-811.
  • 2Tabara H, Grishok A, Mello CC. RNAi in C. elegans: soaking in the genome sequence [J]. Science. 1998, 282(5388): 430-431.
  • 3Napoli C, Lemieux C, Jorgensen R. Introduction of a chimeric chalcone synthase gene into petunia results in reversible cosuppression of homologous genes in trans [J]. Plant Cell, 1990, 2(4): 279-289.
  • 4Cogoni C, Romano N, Macino G. Suppression of gene expression by homologous transgenes [J]. Antonie Van Leeuwenhoek, 1994,65(3): 205-209.
  • 5过七根,陈付学,宋红生,文铁桥.RNA干涉的研究进展[J].河南科技大学学报（农学版）,2004,24(1):5-9. 被引量：1
  • 6Hammond S M. An RNA-directed nuclease mediate posttranscriptional genesilencing in drosopilace cells [J]. Nature, 2000, 404:293-296.
  • 7Hamilton A, Voinnet O, Chappell L, et al. Two classes of short interfering RNA in RNA silencing [J]. EMBO J, 2002, 21 (17):4671-4679.
  • 8Clemens J C, Worby C A, Simonson-Leff N, et al. Use of double -stranded RNA interference in drosophila cell lines to dissect signal transduction pathways [J]. Proc Natl Acad Sci USA, 2000,97(12): 6499-6503.
  • 9阚学峰,高英堂,杜智.RNA干扰作用机制研究进展[J].国外医学（肿瘤学分册）,2004,31(4):246-248. 被引量：6
  • 10Svoboda P, Stein P, Hayashi H, et al. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference [J]. Development, 2000, 127(19): 4147-4156.

二级参考文献32

  • 1Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 1998,391 (6669): 806-811.
  • 2Billy E, Brondani V, Zhang H, et al. Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines. Proc Natl Acad Sci USA,2001,98(25): 14428-14433.
  • 3Elbashir SM, Lendeckel W, Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev, 2001, 15(2): 188-200.
  • 4Provost P, Dishart D, Doucet J, et al. Ribonuclease activity and RNA binding of recombinant human Dicer. EMBO J, 2002, 21(21) :5864-5874.
  • 5Bernstein E, Caudy AA, Hammond SM,et al. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature,2001,409(6818) :363-366.
  • 6Zeng Y, Cullen BR. Sequence requirements for micro RNA processing and function in human cells. RNA, 2003,9(1 ): 112-123.
  • 7Hutvagner G, McLachlan J, Pasquinelli AE, et al. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science, 2001,293(5531) :834-838.
  • 8Hutvagner G,Zamore PD. A microRNA in a multiple-turnover RNAi enzyme complex. Science, 2002,297(5589) :2056-2060.
  • 9Zeng Y, Cullen BR. RNA interference in human cells is restricted to the cytoplasm. RNA, 2002,8(7) :855-860.
  • 10Hammond SM, Boettcher S, Caudy AA, et al. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science,2001,293(5532): 1146-1150.

共引文献12

同被引文献42

  • 1邱达泰,黄芝,欧阳高亮,鲍仕登.肿瘤血管生成促进因子和抑制因子[J].中国药物与临床,2005,5(7):485-488. 被引量：6
  • 2李云龙,秦鹏春,徐立斌,梁建广.小鼠早期胚胎体外培养的实验研究[J].东北农学院学报,1990,21(2):192-195. 被引量：1
  • 3李丽文,朱延明,李杰,柏锡,才华.RNAi技术在植物功能基因组学中的研究进展[J].东北农业大学学报,2007,38(1):119-124. 被引量：24
  • 4Kawamura Y, Saito K. Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells[J]. Nature , 2008, 453(7196) :793 - 797.
  • 5Tam OH, Aravin A, Stein P, et al. Pseudogene - derived small interfering RNAs regulate gene expression in mouse oocytes [ J ]. Nature, 2008, 453(7194) : 534 -538.
  • 6Li R, Li Y. SOAP: short oligonucleotide alignmen program [ J ]. Bioinformatics, 2008, 24(5):713-714.
  • 7DeMarco R, Machado AA, Bisson - Filho A W,et al. Identification of 18 new transcribed retrotransposons in Schistosoma mansoni [ J ]. Biochemical and biophysical research communications, 2005, 333 ( 1 ) : 230 - 240.
  • 8Lander ES, Linton LM, Birren B, et al. Initial sequencing and analysis of the human genome [ J ]. Nature, 2001, 409 ( 6822 ) : 860 -921.
  • 9Waterston RH, Lindblad -- Toh K, Rogers J , et al. Initial sequencing and comparative analysis of the mouse genome [ J ]. Nature, 2002, 420(6915) : 520 -562.
  • 10Ghildiyal M, Seitz H, Li C, et al. Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells [ J ]. Science , 2008, 320(5879) : 1077 - 1081.

引证文献4

二级引证文献4

东北农业大学学报
2005年 第3期

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