超声评价贝那普利与氯沙坦联合应用对自发性高血压大鼠心功能的影响

The evaluation of the combined effects of benazepril and losartan on cardiac function of spontaneous hypertension rats (SHRs) by echocardiography

目的超声评价贝那普利或氯沙坦单用与合用对自发性高血压大鼠(SHR)心脏结构及功能的影响。方法选用55周龄WKY12只,同龄SHR48只,随机分为SHR对照组,贝那普利组(10mg·kg-1·d-1),氯沙坦组(30mg·kg-1·d-1),贝那普利(10mg·kg-1·d-1)+氯沙坦(15mg·kg-1·d-1)组。监测动脉收缩压及体重、心率等基础指标;超声心动图进行左室腔径各指标测量及二尖瓣血流频谱分析;比较各组大鼠的左心室质量指数(LVMI);光镜观察心肌组织结构改变。结果各药物干预组SHR的收缩压低于SHR对照组(P<0.01);各用药组能不同程度降低LVPW、IVS和升高LVEDD值(P<0.01),且能不同程度升高LVEF、E/A值(P<0.01),均以药物合用组更为显著(P<0.01);另外,各用药组均能改善心肌细胞的排列、肥大、凋亡及坏死,改善程度:药物合用组>氯沙坦组>贝那普利组。结论贝那普利和氯沙坦联合治疗SHR对其心脏结构和功能的改善作用优于单用。

Objective To explore the combined effects of benazepril and losartan on configuration and function of the spontaneous hypertension rats' (SHRs) heart by echocardiography. Methods 12 WKY rats were selected as controls,and 48 SHRs aged 55 week-old were randomly divided into SHR-control group, benazepril group (10 mg·kg^(-1)·d^(-1)), losartan group (30 mg·kg^(-1)·d^(-1)) and benazepril (10 mg·kg^(-1)·d^(-1)) +losartan (15 mg·kg^(-1)·d^(-1)). SBP, heart rate and body weight was monitored; left ventricular cavity indexes and mitral valve blood folw spectrums were recorded with echocardiography;left ventricular mass index (LVMI) of each group were compared; myocardial pathologic changes were observed with light microscope. Results SBP of drugs intervention groups was lower than that in SHR-control group (P<0.01). LVPW and IVS thickness decreased and LVEDD, LVEF and E/A value increased in drugs intervention groups (P<0.01), especially for the combination group (P<0.01). Otherwise, array, hypertrophy, apoptosis and necrosis of cardiac muscle cells were improved with degrees in turn combination group>losartan group>benazepril group. Conclusions The improvement roles of combinative application of benazepril and losartan in cardiac configuration and function should be better than that of monotherapy.