TORCH与优生:Ⅰ.应用聚合酶链反应法辅助诊断巨细胞病毒性婴儿肝炎综合征

DETECTION OF HUMAN CYTOMEGALOVIRUS IN URINE FROM INFANTS SUFFERING FROM INFANT HEPATITIS SYNDROME BY POLYMERASE CHAIN REACTION

已知巨细胞病毒是婴儿肝炎综合征的常见病原之一,但临床生存诊断困难,且没有确切感染率的报道。根本原因在于,过去没有一种迅速、准确的辅助诊断方法。我们利用基因数据库资料,设计了两对引物,并建立了聚合酶链反应法检测巨细胞病毒技术。该技术反应时间短,结果准确可靠,无交叉反应。我们检测了138例临床诊断为婴儿肝炎综合征的患儿,其中75例检出尿中带病毒,阳性率为66.96％。对照组正常婴儿34名,仅5名尿中查到病毒,阳率性为14.70％。具显著差异(P<0.01)。实验证明,聚合酶链反应法辅助诊断巨细胞病毒感染性婴儿肝炎综合征可靠、可行。数据表明,巨细胞病毒感染在婴儿肝炎综合征中占首要地位。

The urines of 112 infants suffering from infant hepatitis syndrome were analyzed for detectio of human cytomegalovirus by PCR. The positive rate was 66.96%. In 34 normal infants, the positive rate was 14.70%. The positive rate of infants suffering from infant hepatitis syndrome was much higher that of normal infants (P<0.01) . It is evidenced that human cytomegalovirus is one of the most important pathogens of infant hepatitis syndrome. Detection of HCMV in urine from infants suffering from IHS is a rapid, sensitive, stable and convenient method in diagnosing IHS.