利用聚合酶链反应检测122例结核病患者标本中结核菌的研究

Detection of Mvcobacterium Tuberculosis in the sam-ples of 122 TB patients using polymerase chain reactionteachnique

对１２２例门诊及住院结核病人的痰、脓汁等标本进行聚合酶链反应（ＰＣＲ）和直接涂片荧光镜检，培养检查结核菌，结果表示：ＰＣＲ阳性率为５６．９％（５８／１０２），培养阳性率为１４．７％（１５／１０２），直接涂片荧光镜检为２３．５％（２４／１０２）。说明ＰＣＲ特异性好，敏感性强，与临床符合率较高。ＰＣＲ比培养法大大节省时间，为结核病的诊断和鉴别赢得了时间，是一种可以推广的好方法。

y means of polymerase chain reaction (PCR)rechnique, direct smear fluorescence microscopy andbacterial culture, the sputa and purulent secretions of122 TB patients were examined to detectmycobacterium tuberculosis. The results were as fol-lowing, the positive rate of PCR was 56.9% (58 / 102).The positive rate of direct smear fluorescencemicroscopy was 23.5% (24 / 102) and that of bacterialculture was 14.7% (15 / 102). Our investigation showsthat PCR technique is sensitive. The positive rate ofPCR is largely consistent with the clinical diagnosis.Compared with bacterial culture, PCR is a time-sav-ing practice. It is a recommendable method indiagnosis of TB.