摘要
目的 观察柯里拉京(Corilagin)对人胰腺癌Bxpc - 3细胞的抑制作用。方法 采用MTT法和流式细胞仪技术。结果 Corilagin显著抑制了Bxpc- 3细胞的增殖,且呈时间-剂量依赖性;流式细胞仪分析结果显示,Corilagin主要作用于细胞周期的S期,同时也作用于G2 -M期。并且有凋亡峰出现。结论 Corilagin可有效抑制Bxpc - 3细胞增殖,阻滞细胞于S期和诱导凋亡可能是其作用机制之一。
Objective To study the inhibition effect of Corilagin on human pancreatic cancer Bxpc-3 cells. Methods Adopted MTT assay and Flow Cytometry techniques. Results The proliferation of Bxpc-3 cells were inhibited apparently by Corilagin,and the inhibition effect was time and dosage dependence,the flow cytometric data showed:cell cycle was blocked mainly in S phase, synchronously corilagin also affected on the G 2-M phase. Conclusions Corilagin can inhibit the proliferate of Bxpc-3 cells notablely,the S phase arrest and the induction of apoptosis would be a very important mechanism of the effect.
出处
《锦州医学院学报》
2005年第3期25-27,共3页
Journal of Jinzhou Medical College
关键词
柯里拉京
胰腺癌
细胞周期
凋亡
Corilagin
pancreatic cancer
cell cycle
apoptosis
