MIF-173位点单核苷酸多态性与我国胃癌关系的研究

Association of macrophage migration inhibitory factor gene-173 locus polymorphism and gastric cancers in China

目的:研究巨噬细胞移动抑制因子(MIF)基因-173G/C位点单核苷酸多态性在中国胃癌高发区和低发区普通人群和胃癌患者中的分布,并探讨其基因多态性与我国胃癌的关系。方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测年龄和性别匹配的广东省104例普通人及104例胃癌患者和陕西省普通人和胃癌患者各102例的MIF-173位点单核苷酸多态性。结果:在胃癌高发区,胃癌患者携带MIF-173C/C的频率明显高于普通人群(28.8%vs15.4%,χ2=5.47,P<0.05,OR=2.23,95%CI=1.13-4.41),MIF-172C/C基因型增加Hp感染后胃癌发生的危险性(χ2=5.27,P<0.05,OR=8.0,95%CI=1.27-19.72)。在胃癌低发区,胃癌患者MIF-173C/C基因型的频率与正常对照组无明显差异(31.4%vs27.5%,χ2=0.38,P>0.05)。结论:MIF-173C等位基因可能与我国汉族人群胃癌发生有关。

AIM: To study the relationship between MIF gene -173 locus polymorphism, helicobacter pylori (Hp) infection, and gastric cancer in high prevalent (Shanxi) and low prevalent (Guangdong) regions in China. METHODS: Genomic DNA was extracted from peripheral blood of 104 healthy controls, 104 gastric patients from Guangdong and 102 healthy volunteers, and 102 gastric cancer patients from Shanxi. Polymorphism of MIF-173 locus was analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). RESULTS: In high prevalence region, the number of patients who carrying with MIF -173 C/C is much higher than those of healthy controls (28.8% vs 15.4%, χ~2=5.47, P<0.05, OR=2.23, 95% CI=1.13-4.41), the existence of MIF -173 C/C can significantly increase the risk of gastric cancer occurrence after Hp infection (χ~2=5.27, P<0.05, OR=8.0,95% CI=1.27-19.72). In the low prevalence region, the frequency of MIF -173 C/C locus was slightly higher among patients with gastric cancer than healthy controls ,but significant statistics can't be obtained (31.4% vs 27.5%, χ~2=0.38, P>0.05). CONCLUSION: The C genotype of MIF -173 locus may be associated with the risk of gastric cancer in China.