摘要
目的:探讨腹腔振摇对腹膜表面层和腹膜功能的影响。方法:将12只雄性SD大鼠随机分成对照组(n=6)和振摇组(n=6);振摇组大鼠麻醉后经腹部插入静脉留置针,由留置针管注入25mL含[125I]-白蛋白的4.25%的葡萄糖透析液,在电摇床(100r/min)上行腹膜动力学试验;对照组大鼠则行常规腹膜动力学试验。腹膜动力学试验完毕后,取对照组和振摇组大鼠的腹膜组织,经含1%四氧化锇(OsO4)的氟化碳(FC75)固定液固定后,行电镜检查,经Bouin液固定后行常规病理检查(HE染色)。结果:振摇组大鼠的腹膜通透性显著高于对照组,表现为腹腔液体重吸收率明显增加,经毛细血管的超滤率明显下降,淋巴重吸收率明显增加,净超滤量明显下降,小分子溶质转运明显增高。电镜检查结果显示,振摇组的腹膜表面层明显薄于和疏松于对照组,腹膜组织常规病理学检查两组无明显差异。结论:腹腔振摇使腹膜通透性增高,同时破坏了腹膜表面层,提示腹膜表面层在控制腹膜通透性中起重要作用。
AIM: To investigate the effect of peritoneal vibration on the peritoneal permeability and the peritoneal surface layer. METHODS: Peritoneal transport rate was examined in twelve male SD rats. Six (S group) were put on an electronic shaker and the other six were used as control (C group). After that, the peritoneum was examined by electron microscopy (EM). RESULTS: The net ultrafiltration volume (NUF) in the S group was lower than that in the C group. This difference in NUF was due to both a significantly higher peritoneal fluid absorption rate and a significantly lower transcapillary ultrafiltration rate in S group as compared to C group. The peritoneal direct lymphatic absorption rate was higher in S group. The transport rates of small solutes were also significantly higher in S group. EM showed that the thickness of the peritoneal surface layer was significantly decreased in S group. CONCLUSION: Our results suggest that the peritoneal surface layer may be an important layer in modulating the peritoneal transport rate.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第6期1163-1166,共4页
Chinese Journal of Pathophysiology
基金
中山医科大学"211"工程基金资助项目(No.98151)
中山医科大学校基金(No.072009)资助项目
关键词
振动
腹膜透析
腹膜表面层
Vibration
Peritoneal dialysis
Peritoneal surface layer