摘要
目的研究节律基因mPeriod2(mPer2)对肿瘤细胞的生长抑制及诱导分化凋亡的作用。方法体外培养Lewis肺癌细胞,使用脂质体包裹法将真核表达质粒pcDNA3.1为基础的mPer2表达质粒(pcDNA3.1mPer2)转导入Lewis肺癌细胞内;以免疫组化及流式细胞技术检测mPer2表达;通过流式细胞技术检测稳定转染的mPer2阳性表达的Lewis肺癌细胞的生长及凋亡。结果mPer2表达质粒(pcDNA3.1mPer2)转染的Lewis肺癌细胞表达PERIOD2蛋白;与对照组比较,其增殖指数下降及凋亡率增加。结论节律基因mPer2可以抑制肿瘤细胞的生长,增加肿瘤细胞的凋亡率。
Objective To investigate the role of circadian gene mPeriod2 (mPer2) on tumor proliferation and apoptosis. Method The eukaryotic mPer2 expression vector (pcDNA 3.1-mPer2) based on pcDNA 3.1 was transfected into the cultured Lewis tumor cell by liposome method in vivo. The expression of mPer2 in transfected Lewis tumor cell was detected by immunohistochemistry and flowcytometry. The stable transfected cell line was screened by G418 and its proliferation and apoptosis was examined by flowcytometry. Result Expression of PERIOD2 protein in pcDNA 3.1-mPer2 transfected Lewis tumor cell was proved by immunohistochemistry and flowcytometry. Flowcytometry result of mPer2 expression Lewis tumor cell showed less proliferation and high rate of apoptosis. Conclusion mPer2 expression can suppress the proliferation of tumor cell and increase the apoptosis of it.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2005年第3期222-223,共2页
Space Medicine & Medical Engineering
基金
国家自然科学基金(30470623
30470684)
