牙周病患者血清及龈沟液特异抗体水平与龈下细菌量的关系

Relatiouship of serum and gingival crevicular fluidantibody levels with the amount of subgingival homol-ogous bacterium in patients with periodontul disease

采用ＥＬＩＳＡ检测３９名不同类型牙周病患者的血清及其１５６颗患牙的龈沟液（ＧＣＦ）中抗牙龈叶琳菌（Ｐｇ）抗体（ＩｇＧ）水平，并用间接免疫荧光法检测龈下菌斑中的Ｐｇ，观察Ｐｇ检出量、血清（Ｓｒ）和ＧＣＦ抗体水平、二者抗体水平比值（ＧＣＦ／Ｓｒ比值）以及与临床指数间的关系。结果显示，Ｐｇ检出量与疾病类型、临床指数呈正相关，与ＧＣＦ抗Ｐｇ抗体及ＧＣＦ／Ｓｒ比值呈负相关。初步证实龈下菌斑中细菌量增多，会使ＧＣＦ中相应抗体水平下降，从而使ＧＣＦ／Ｓｒ比值降低。ＧＣＦ抗Ｐｇ抗体水平、ＧＣＦ／Ｓｒ比值及龈下菌斑中Ｐｇ检出量结合起来，有可能成为牙周病活动期的诊断指标。

Abstract39 patients were selected in this study, including12 patients with rapidly progressive periodontitis(RPP) , 17 with adult periodontitis fAP) and 10 withmarginal gingivitis (MG). The levels of antibody toPorphyromonas gingivalis (Pg) in serum (Sr) and ingingival crevicular fluid (GCF) from their 156 teethwere measured by ELISA. The Pg in subgingivalplaque was detected by indirect immunofluorescencetechnique. Data showed different GCF/Sr ratio of an-ti-Pg antibody level between periodontitis patients(loewr than 1) and MG patients (greater than 1).The amount of subgingival Pg was negatively corre-lated with the GCF/Sr ratio, and also positively cor-related with clinical periodontal parameters, i. e,probing depth, attachment loss, plaque index andbleeding index. It was suggested that low GCF/Sr ra-tio of antibody level plus high counts of homologousbacterium might be used as an indicator of periodontaldisease activity.