二烯丙基三硫对MGC-803细胞凋亡的影响

The Effect of DATS Inducing Apoptosis of MGC-803

目的 探讨二烯丙基三硫(diallydisulfide ,DATS)诱导人胃癌MGC - 80 3细胞凋亡及作用机制。方法 光学显微镜观察凋亡形态,流式细胞术检测凋亡及细胞内活性氧水平。结果 1 2mg LDATS作用于胃癌MGC - 80 3细胞2 4小时后,在光学显微镜下出现了典型的凋亡细胞形态学改变,流式细胞仪检测结果提示,活性氧水平升高,出现明显的亚G1峰。用抗氧化剂NAC预处理细胞,能抑制细胞内活性氧水平的升高及DATS诱导的凋亡。结论 DATS诱导胃癌细胞凋亡可能与细胞内活性氧产生增加有关。

Objective To investigate DATS inducing MGC-803 cell apoptosis and its possible mechanism. Methods The apoptotic morphological change was observed under the light microscope. Intracellular reactive oxygen species (ROS) level and the rate of apoptosis were detected by the flow cytometry. Results After MGC-803 cells were treated with 12 mg/L DATS for 24 hours, the typical apoptotic morphological changes were observed under the light microscope, flow cytometry analysis showed that intracellular ROS level and the percentage of apoptosis cell were significantly increased after treating MGC-803 with DATS. Pretreatmentof N-acetyl-l-cystein, a thiol-containing antioxidant, effectively attenuates DATS-induced increasing of intracellular ROS and the consequent apoptotic of MGC803. Conclusion These results suggested that the cellular generation of ROS may play a role in initiating DATS-mediated apoptosis of MGC-803 cells.