三七皂甙单体2A-1-1对高血压患者血小板钙内流的影响

Effects of 2A-1-1 on the Ca^(2+) Influx of Platelets From Essential Hypertension

目的 观察三七皂甙单体2A - 1 - 1对高血压患者血小板钙内流的影响,并探讨其对血小板受体操纵性钙通道(ROC)的作用。方法 采用Fura - 2 Am荧光探针双波长测定细胞胞浆游离钙离子浓度( [Ca2 + ]i)技术,以Nifedipine为对照观察2A - 1 - 1体外对环匹阿尼酸(CPA)介导的高血压患者血小板钙内流变化。结果 高血压患者血小板静息钙和钙内流均高于正常人(P <0 .0 5 ) ;2A - 1 - 1 ( 5、1 0、2 0 μmol L)可抑制CPA介导的高血压患者血小板钙内流(P <0 .0 5或0 .0 1 ) ;Nifedipine( 2 0 μmol L)不能抑制CPA介导的高血压患者或正常人血小板钙内流(P >0 .0 5 )。结论 2A - 1 - 1可能通过抑制高血压患者血小板ROC ,减少钙内流,从而降低其血小板活性。

Objective: To observe the effect of Calcium channel's blocks on Ca 2+ influx of platelets from essential hypertension. And to explore its effect on the ROC(Receptor-dependent Ca 2+ channels) of platelets. Methods Fura-2 fluorescent technique was applied to detect cell [Ca 2+ ]i, and the effects of two types of Calcium channel's blocks on Ca 2+ influx of human platelets induced by CPA differently was observed. Results The resting [Ca 2+ ]i and Ca 2+ influx of platelet in essential hypertension group is higher than that in normal group. Nifedipine can not affect the Ca 2+ influx of platelets from essential hypertension or normal induced by CPA. 5,10 or 20 μmol/L 2A-1-1(purified component from Panax notoginsengs saponins)can refrain the Ca 2+ influx of platelets from essential hypertension induced by CPA. Conclusions 2A-1-1 can block the ROC(Receptor-dependent Ca 2+ channels) of the human platelets from essential hypertension,and can refrain Ca 2+ influx of platelets.