黄山松群体遗传多样性分析

Analysis of the genetic diversity of Pinus taiwanensis populations

利用RAPD分子标记分析了黄山松 1 0个家系的遗传多样性。从 2 0 6个随机引物 (组 )中筛选出 1 7个引物 ,获得 38个多态位点。Shannon多样性指数平均为 4 5 2 5 1 ,Shannon多样性值范围在 0 0 1 0 2～ 0 0 5 0 4。家系 (余姚×文石 )遗传多样性水平最高 ,其Shannon多样性指数为7 965 8。根据这 38个多态位点计算遗传距离 ,进行聚类分析。在遗传距离 0 42处可将 1 0个家系聚类分成 3组。图 2表 3参 1

The genetic diversity of 10 Pinus taiwanensis families are analyzed by RAPD molecular markers.17 primers and 38 polymorphic loci are screened from 206 random oligonucleotide primers.The mean Shannon Wevieis diversity index is 4 525 1,which ranges within 0 010 2～0 050 4.The genetic diversity level of the family(Yuyao×Wenshi)is the highest and its Shannon Wevieis index is 7 965 8.Genetic distance is calculated according to 38 polymorphic loci and clustering analysis is carried out.10 clustering families can be divided into 3 groups at the point of the genetic distance 0 42.