胸水军团菌抗体检测的探讨—附军团菌肺炎合并胸水一例报告

Detection of Antibodies against Legio-nella pneumophila from Pleural Effusion──a case report of Legionnaire′s pneu-monia with pleural effusion

我们应用试管凝集法及酶联免疫吸附试验方法检测了５０例非军团菌感染病人胸水中嗜肺军团菌１、６型抗体及病人血清中相应抗体。发现在这些普通病人胸水中也存在一定的嗜肺军团菌抗体滴度，并计算出这些抗体的９９％上限滴度（Ｘ＋２．３２６Ｓ）。为今后检测嗜肺军团菌感染病人的胸水提供了一个参比对照指标。实验后期，我们检测了一例军团菌肺炎病人，其胸水中Ｌｐ１型抗体滴度远远超过了该上限值，并高于其自身血清中相应抗体。

sing TAT and ELISA,50 samples of pleu- ral effusion and sera from patients infected with non-Legionella pneumophila were detec- ted for antibodies against Legionella pneumo- phila serogroups 1 and 6,respectively.The average titre and upper limit value(99%,X+ 2.326S)calculated,In addition,a pleural effusion and a serum from a patient with Legionnaire′s pneumonia were also detected by TAT andELISA.The result showed that the titre ofthis antibody in his pleural effusion was notonly over the upper limit,but also higher thanthat in his serum.