聚合酶链反应对小儿肺炎多病原检测及临床意义

The Detection of Ploy Pathogens in Children with Pheumonia with PCR and Its Clinical Value

目的 了解我区病毒性肺炎的病原以及病原之间的关系、病原与临床的关系。方法 对 2 0 0例肺炎患儿鼻咽部分泌物用PCR法检测呼吸道合胞病毒、腺病毒 ,流感Ⅰ、Ⅱ型 ,肺炎支原体。结果 呼吸道合胞病毒 4 8.2 %、腺病毒 2 3.4 %、流感Ⅰ型 2 3.4 %、流感Ⅱ型 2 .1%、支原体 2 .8% ,总阳性率 70 .5 % ,6个月以内以呼吸道病毒及流感Ⅰ型为主 ,6个月～ 3岁以腺病毒及呼吸道合胞病毒为主 ,年长儿则支原体感染率增加。结论 采用PCR法进行的病毒检测提高阳性率。我区小儿肺炎常见病原以呼吸道合胞病毒为主 ,腺病毒及流感病毒次之 。

Objective To investigate the pathogens of virus pneunia in our city and the relations between pathogens and pathogens,pathogens and clinic. Methods 200 children with pneumonia were selected and their nasopharyngeal discharge was checked with PCR to find respiratory syncytial virus,adenovirus,influenza virus Ⅰ,influenza virus Ⅱ,mycoplasmal pneumoniae.Results The respiratory syncytial virus was 8.2%, adenovirus 23.4%,influenza virus Ⅰ 23.4%,influenza virus Ⅱ 2.1%,mycoplasmal pneumoniae 2.8%,the total positive rate 70.5%. According to children under six months, the majority viruses were respiratory syncytial virus and influenza virus Ⅰ. According to children from six months to three years, adenovirus and respiratory syncytial virus accounted for the majority. According to elder children, the infection rate of mycoplasmal pneumoniae increased. Conclusion Using PCR to check ploy pathogens is a good way to improve the positive rate. The normal pathogens of children with pneumoria in our city were major in respiratory syncytial virus, adenovirus and influenza virus were the second, mycoplasmal pneumoniae made up some percentages.