应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体

Detection of Anti-BB Antibodies in Human Sera by VIDAS System

莱姆病（Ｌｙｍｅｄｉｓｅａｓｅ）是最近十年来才被认识的一种蜱媒传染性流行病。Ｌｙｍｅ病的血清学诊断方法有间接荧光免疫法（ＩＦＡ）．酶免疫试验（ＥＩＡ），免疫印迹法（ＷＢ）。梅里埃生物公司（Ｂｉｏｍｅｒｉｅｕｘ）推出ＶＩＤＡＳ免疫诊断系统能够自动定性和半定量地检查Ｌｙｍｅ病的抗伯氏包柔螺旋体抗体（抗ＢＢ抗体）．本法结合了酶和荧光免疫两种方法。硷性磷酸酶标记二抗的底物是４ｍｅｔｈｙｌｕ－ｍｂｅｌｌｉｆｅｒｙｐｈｏｓｐｈａｔｅ，底物在碱性磷酸酶的催化下转变成４－ｍｅｔｈｙｌｕｍｂｅｌｌｉｆｅｒｏｎ，该产物在３６５ｎｍ光激发下可发出４５５ｎｍ的荧光，光强度被ＶＩＤＡＳ中的光扫描器测定出来。我们用ＶＩＤＡＳ检测了体检标本８３例，阳性８份（９．６％）；检测临床为结核病的标本２０份，无一例阳性；临床诊断为结节病或可疑结节病标本４６份，阳性４份（８．７％）；各种眼科疾病５７份，阳性１３份（２２．８％）；口唇疾病１５份，阳性４份（２６．７％）；其他标本１７份，阳性２份（１１．８％）；检测了军事医学科学院（军科院）微生物流行病研究所采自新疆、黑龙江省可疑Ｌｙｍｅ病病人的标本１６份，检出阳性１２份（７５．０％）。笔者用ＶＩＤＡＳ?

yme disease is an infectious disease just known in the latest decade. The sera of people from various origins were detected for Anti-BBantibodies(IgM+ IgG)by VIDAS system.The results are as follows:of 83 sera from the health-cared people,8 were positive(9.6 %);of 20 sera from the patients clinical diagnosised as tuberculosis,none was positive; of 46 sera from the patients clinical diagnosised or suspected as node diseasas,4 were positive(8.7%);of 57 sera from the patients with various ophthal-mologic diseases,13 were positive(22.8m%);of 15 eera trom the patients with stomatologic disea-ses,4 were positive(26.7%):of 17 sera from the patients with various diseases including dermo-myositis,liver and spleen swelling. undefhited febrile diseases:lung cancer,lung shadow,etc,),2,were positive(11.8%);of 16 sera from the people highly suspected with lyme disease living in epidemic areas of Xin Jiang andHei Long Jiang provinces.12 were positive(75%)、The same 166 sera samples were detected at the same time by VIDAS System and FA,24 were positive by VIDAS System (14.5%),and 131 positive by IHA(79%)the accordance rate was only 32.7%.