临床分离286株革兰氏阴性菌耐药谱及R－质粒检测

Assay of R-Plasmid and Antibiotic Resis-tance Spectrum in 286Strains of Gram-Negative Bacteria

本文报道２８６株临床分离Ｇ－菌对１０种抗菌素的药敏测定及Ｒ－质粒检测结果。实验发现目前主要流行的耐药菌为绿脓杆菌、大肠杆菌、克雷伯氏菌，耐药谱为Ａｐ、Ｃｂ、Ｃｍ、Ｇｍ、Ｆｂ、ＣＰＶ型，２８６株菌中２７２株对不同抗生素耐药（９５．１％）；１７８株菌为多重耐药菌（６２．２％），５３．３％的耐药菌携带Ｒ－质粒，１３．３％的耐药标记为整传递，８６．７％的耐药标记为部分传递。有意义的是来自不同时间、不同病房的病人分离菌却具有极相似的耐药谱及Ｒ－质粒传递谱，这些患者多为引流术及术后感染者。

his paper reports the results of the sensi-tivity test to 10 antibiotics and the assay of R-plasmid on 286 strains of Gramnegative bacteriaisolated from hospital. It was found that themain prevalent resistant bacteria are pseudomo-nas aeruginosa, Escherichia coli and Klebsiel-la pneumoniae. Their major antibiotic resistancespectrum is:Ampicillin, Cabenicillin, Chloram-phemicol.Centamicin, Furbenicillin.The results showed that 272 of 286 strains resisted to 1-9 antibitoics (95.1%).One-hundred and seventy-eight of 286 strains resisted to more than 4 antibiotics(62.2%).Cne-hundred and fifty-one of 286 strains carried R-plamid in resistant bacteria (53.3%). 13.3% resistance markers were transferred with the whole R-fac-tor.But 86.7& resistance markers were transfer-red with only a part of R-factor.