脉冲电场对悬浮中国红豆杉细胞的影响

Effects of Pulsed Electric Field on Suspension Cultures of Taxus chinensis

以中国红豆杉悬浮细胞为植物细胞的模型,研究了低频脉冲电场对植物细胞生长和次生代谢的影响.不同生长时相的红豆杉细胞在不同时间的脉冲电场作用下,其生长和紫杉烷积累的情况表现出明显差异.对数前期的细胞经30min的电场诱导后,细胞生长没有明显变化,但紫杉烷的含量提高了近30%.并发现,脉冲电场和补糖的组合策略能更有效地促进细胞积累紫杉烷.

Suspension culture of Taxus chinensis was used as a model plant cell system to study the effects of pulsed electric field (PEF) on cell growth and the biosynthesis of secondary metabolites. Different exposure time of PEF had different effects on Taxus chinensis cells in different cell growth phases. Significant intracellular enhanced accumulation of taxuyunnanine C (Tc, a bioactive secondary metabolite) was observed by exposing the cells in early exponential growth phase to a 30 min PEF. The Tc content increased by 30% after exposure to PEF. without any loss of dry cell weight compared with that of the control. Moreover, combination of PEF with sucrose feeding showed a great synergistic accumulative effect on secondary metabolite production.