双氢青蒿素治疗对卡氏肺孢子虫肺炎大鼠脾细胞上清液一氧化氮水平的影响

Effects of treatment with dihydroartemisinin on NO in the supernatant of spleen cells in rats infected with Pneumocystis carinii pneumonia

目的 研究双氢青蒿素治疗对卡氏肺孢子虫肺炎 (PCD)大鼠脾细胞上清液一氧化氮 (NO)水平的影响。方法 以醋酸可的松皮下注射Wistar大鼠 ,建立卡氏肺孢子虫肺炎动物模型 ,用 60mg/kg双氢青蒿素治疗实验大鼠 ,杀鼠取肺 ,胶原酶消化法分离脾细胞 ,脂多糖 (LPS)刺激培养 72h ,用NO试剂盒检测大鼠脾细胞上清液NO活性 ,同时设有感染组和正常对照组。结果 感染组和治疗组大鼠NO水平高于正常对照组 ,治疗组NO水平低于感染组。结论 卡氏肺孢子虫 (Pc)感染可能引起大鼠脾细胞分泌高水平NO ,发挥杀伤Pc作用 ,同时加重宿主组织炎症反应 ,抑制宿主的免疫应答 ;经双氢青蒿素治疗后 ,PCP大鼠脾细胞分泌NO降低 ,组织炎症反应减轻 。

Objective To study the effects of treatment with dihydroartemisinin(DHA) on NO in the supernatant of spleen cells in rats infected with Pneumocystis carinii pneumonia(PCP).Methods Wistar rat model of PCP was established by subcutaneous injection with cortisone acetate, experimental rats were treated by 60 mg/kg DHA and killed to obtain spleens, spleen cells were separated by collagenase digestion and cultured by lippolysa ccharide(LPS) stimulation in 72 h,the level of NO in the supernatant of spleen cells was detected by NO kits,infected and normal rats served as controls.Results The concentration of NO in the infection group and treatment group was higher than that in the normal control,but that in the treatment group was lower than that in the infection group.Conclusions Spleen cells of PCP rats may secrete high level of NO, give play to killing Pc, exacerbate tissue inflammation and inhibited host's immune responses, but these effects were reversed after DHA therapy.