α-淀粉酶法制备小麦胚芽蛋白的研究

Research of alpha amylase hydrolyzed wheat germ protein to product wheat germ protein

小麦胚芽用超临界CO2脱脂,用α-淀粉酶水解麦胚蛋白提取液中的淀粉,酸沉淀麦胚蛋白,使麦胚蛋白含量和得率均较碱溶酸沉淀法明显提高,分别达到94.5%和81.36%,用高效毛细管电泳鉴定小麦胚芽蛋白的纯度,SDS-PAGE电泳测定小麦胚芽蛋白的分子量。

Wheat germ was defatted by supercritical dioxide, starch in the extract of wheat germ protein was hydrolyzed by alpha amylase,wheat germ protein was deposited by acid,the content of wheat germ protein and obtain ratio was reached 94.5% and 81.36%.The purity degree of wheat germ protein was charactered by high performance capillary electrophoresis, the molecule weigh was determined by SDS-PAGE electrophoresis.