新鬼臼毒素衍生物GP_7对膀胱癌的体外实验及临床观察

Chemosensitivity testing of podophyllotoxin derivative GP_7 in human bladder transitional epithelial carcinoma cell line BIU-87 and human bladder trnasitional carcinoma

为探讨第三代鬼臼毒素衍生物ＧＰ_７对膀胱癌的疗效，经体外实验及临床试用研究并与ＶＰ_（１６）作对照观察。各项指标表明：ＧＰ_７较ＶＰ_（１６）有特殊的超微结构变化，对癌细胞有更强的杀伤力。并作出了二者在细胞内作用机理不尽相同的推论。在初步临床试用ＧＰ_７瘤体注射的９例患者中，７例标本组织改变与体外实验大体相符，２例肿瘤消失，其基底部组织未见癌细胞。

GP_7 was studied, compared with VP_(16) and the controls so as to evaluate its effect on the human transitional bladder tumor cells. In vitro studies, GP_7 was found to be more powerful than VP_(16) as to the cytotoxicity against BIU-87 cells. Special variation in cytoplasm was observed in GP_7 treated cells and therefore the mechanism of GP_7 action on cells was believed to be different from that of VP_(16). In vivo studies, intratumor injectiom of GP_7 was carried out in 9 cases of bladder tumor in 7 of them similar changes of tumor tissue as observed in vitro were found while in the other 2 the tumor totally disappeared.