成龄大鼠肾上腺同种异体肾被膜下种植的实验研究

An experimental study on adrenal glands homotransplantation to the subrenal capsule in adult rats

将１２０只成龄雌性Ｗｉｓｔａｒ大鼠双侧肾上腺全切除后，将异体肾上腺组织块种植于肾被膜下（ＳＲＣ）。结果显示：种植组织可存活及再生。单纯种植组术后早期种植组织有淋巴细胞浸润，而应用环孢素Ａ（ＣｓＡ）能抑制排斥反应，再联用ＡＣＴＨ则可促进移植体再生。一定程度再生后停用ＣｓＡ，种植体可长期存活，并发挥正常的分泌功能，其效果与吻合血管的异体肾上腺移植相同。本法技术简单，取材方便，为临床肾上腺同种异体移植提供一简便有效的方法。

adult female Wistar rats undergone bilateral adrenelectomy were diveded to four groups:(A)simple bilateral adrenalectomy;(B)bilateral adrenalectomy with pieces of adrenal glands alloimplanted to the subrenal capsule (SRC);(C)the same with group B followed by institution of cyclosporing A(CsA) from the operative day to the 14th postoperative day;(D) group C plus ACTH given from 3rd to 31st postoperative day. Five months follow-up showed that all the grafts survived, regenerated and the serum corticosteron levels were in correspondence with histological features. This result showed SRC is an ideal site for adreal homotransplantation. Varying degrees of lymphocytyc infiltration were present in the implants in group B on the 2nd to 21st postoperative day, the fragment in groun C and D in which CsA was given did not show any lymphocytic infiltration, even after CsA was discussed. ACTH instituted in group D remarkably enhances the regenerative speed of the grafts After CsA and ACTH were stopped, no rejection episode appeared and the adrenal grafts survived well. The result has been similar to clinical homotransplantation of adrenal glands with vasculas anastomosis.The technigue is simple and the adrenal gland used for homotransplantation is easy to obtain.