HESW与抗癌药物对膀胱癌细胞C－myc癌基因表达的影响

The influence of HESW and antitunor-drug on the expression of C-myc oncogene in human bladder tumor cell line.

利用国产液电碎石机（ＥＳＷＬ－Ⅲ／２Ｍ型）产生的高能冲击波和１０－羟基喜树碱单纯或联合处理人膀胱癌细胞系（ＢＴ５６３７）。实验分对照组，ＨＥＳＷ组，药物组和联合组。冲击波条件：２００ＳＷ（１０ｋＶ，６０次／ｍｉｎ），药物浓度（０．１μｇ／ｍｌ），水温３７℃。采用α－３２Ｐ－ｄＣＴＰ标记Ｃ－ｍｙｃ癌基因探针，通过Ｎｏｒｔｈｅｒｎ印迹杂交技术，分析四组癌细胞Ｃ－ｍｙｃ癌基因表达。结果表明：对照组Ｃ－ｍｙｃ癌基因表达明显，其转录产物为２．７ｋｂ，而实验各组Ｃ－ｍｙｃ癌基因表达完全被抑制。结论：ＨＥＳＷ，１０－羟基喜树碱及其联合对人膀胱癌细胞的生长抑制作用与Ｃ－ｍｙｃ癌基因表达有密切关系。

Human bladder tumor cell line (BT5637) was managed by HESW(high energy shock waves,electrohydrulically generated, ESSL-Ⅲ/2M type) alone or in combination with 10-hydroxy camptothecimun(an anti-tumor drug).The experiment includes a comtrol,a single HESW,a singal drug and a combination group.200sw(10kV, 60time/min)and 0.1ug/ml 10-hydroxy camptothecinum were given to the HESW, drug and combination group respectively. The expression of C-myc oncogene in these groups was analysed by northern blot hybridezation with a-￣(32)p-dCTP labeled C-myc gene probe. The Result showed that C-myc oncogene expression in the cotrol group has been very obvious,Its transcription product being 2.7kb. But the C-myc oncogene expression in the experimental groups were inhibited completely.