人巨细胞病毒感染对肾移植受者外周血T细胞亚群的影响及其临床意义

Effects of HCMV infection on circulating T cell subsets and the clinical sequelae in renal transplant recipients

应用ＰＣＲ技术及ＥＬＩＳＡ法诊断ＨＣＭＶ感染，６５例肾移植受者中感染率为６０％。应用ＡＰＡＡＰ法检测其外周血Ｔ细胞亚群，发现ＨＣＭＶ感染导致ＣＤ４／ＣＤ８值显著降低。ＨＣＭＶ产生的超级免疫抑制导致了严重的临床后果：ＨＣＭＶ感染组革兰细菌、结核杆菌、霉菌、ＨＳＶ及ＶＺＶ等所致的机会感染率明显高于ＨＣＭＶ未感染组；ＡＡＧ发生率明显增高。

In 39 renal transplant recipients HCMV infection was diagnosed by PCR and ELISA. Circulating T cell subsets were assayed by APAAP method, The result indicated that HCMV infection, seriously affected circulating T cell subsets resulting in CD_4/CD_8 decrease of inverse (HCMV infection group: 1.38±0.019 ; noinfection group: 1.98±0.045, P <0.01). The immunosuppressive effects of HCMV infection on recipients had led to serious clinical sequelae, the occurrence of opportunistic infection (gram-bacteria, TB, Fugus, HSV and VZV)being more frequnt in the HCMV infection group than the noinfection group( P <0.01) and so was acute renal allograft glomerulopath ( AAG ) ( P <0.01 ) .