自建血清前列腺特异抗原测定方法的临床应用

Establisbed oneself the detection of serum prostate spe-cific antigen and application in clinical practice

作者建立了血清前列腺特异抗原（ＰＳＡ）的生物素－亲和素酶联免疫（ＢＡ－ＥＬＩＳＡ）测定法并用于临床。测定结果表明，合并前列腺急或慢性炎症患者的血清ＰＳＡ水平显著高于单纯前列腺增生患者。以１０ｎｇ／ｍｌ为界值诊断前列腺癌，其敏感性为６２．９６％，特异性为９１．８１％。我们用自建的ＢＡ－ＥＬＩＳＡ法和进口试剂盒Ｔａｎｄｅｍ－Ｅ法平行测定了良性前列腺增生组的血清ＰＳＡ水平，结果表明ＢＡ－ＥＬＩＳＡ法用于临床比Ｔａｎｄｅｍ－Ｅ法更灵敏、更经济。

AbstractSerum prostate specific antigen(PSA)-the biotin-avidin enzym immunoassay(BA-ELISA)was estab-lished and used in clinical practice.We assayed serumPSA in normal control and prostatic patients. If 10ng/ml was taken as the cutoff value,the positive rate ofprostatic cancer was 62. 96%.The results of determi-nation showed:PSA serum levels of patients with com-plicated BPH(acute prostatitis and chronic prostatitis )were conspicuously higher than the noncomplicatedBPH.In the BPH group,we simultaneously detectedserum levels of PSA by BA-ELISA and Tandem-EPSAImmuno Enzy Metric Assay.The results suggestedBA-ELISA technique is more sensitive and more eco-nomical than Tandem-EPSA Immuno Enzy Metric AS-say for clinical use.