流式细胞计检测人精子顶体及其与精液其他参数的关系

Flow cytometry of human acrosome and its relationship to other semen characteristics

运用流式细胞计及人精子顶体特异性标记物结合异硫氰酸荧光素的花生凝集素检测了３４例精液精子顶体结构的完整性。其中正常生育精液１２例，弱精症精液１２例，畸形弱精症精液１０例。并且进一步检测了２０例精液在体外诱导下精子发生顶体反应的能力，其中正常生育精液１０例，不育精液１０例。结果表明人精子顶体结构的完整性与精于正常形态百分率，存活率，前向运动百分率显著正相关（Ｐ＜０．００１）。不育组精子在体外诱导下发生顶体反应的能力明显低于生育组（Ｐ＜０．０１），说明流式细胞计能用于预测生育力。

Flow cytometry of human sperm acrosome,labelled by combined FITC with arachis hypogaea agglutinin was undertaken to determine the integrity of sperm acrosome for 34 semen samples including fertile,asthenozcospermia, and asthenoteratozcospermia.The ability of induced sperm acrosome reaction was also studied in vitro,including fertile and infertile.The results revealed that a statistical correlation could be demonstrated between the integrity of sperm acrosome on the one hand and the proportion of sperm with normal morphology,vitality and progressive motility on the other(P<0.001).The ability of induced sperm acrosome reaction in vitro was significantly reduced in the infertile men, as compared with the fertile(P<0.001).It is believed that flow cytometry can be used to predict fertility.