应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达

Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction

应用逆转录酶链式反应（ＲＴ－ＰＣＲ）技术，以β－微球蛋白基因为内对照，对２８例术前未接受化疗的膀胱癌标本及１２例正常膀胱粘膜ＭＤ１基因的表达进行了分析，８２．１％（２３／２８）膀胱癌和４１．７％（５／１２）正常膀胱粘膜在１６７ｂｐ处显示ＭＤＲ１基因特异的扩增片段，全部标本在１２０ｂｐ处显示β－微球蛋白基因特异的扩增片段。结果表明，膀胱癌及正常膀胱粘膜存在内源性ＭＤＲ１基因的表达，从而提示膀胱癌患者对化疗药物敏感性的差异可能与其ＭＤＲ１基因的异常表达有关。同时证实ＲＴ－ＰＣＲ技术是一种灵敏的，可靠的ＭＤＲ１基因表达的检测方法。

We have studied the expression of multidrug resistance,(MDR1) gene in 28 specimens from patients with bladder cancer with no prior exposure to chemotheraputic drugs and in 12 normal bladder mucosa specimens by reverse transcription-polymerase chain reaction(RTPCR). MDR1 gene specific 167bp amplified fragments were found in 23 tumor and 5 normal tissues, and β-microglobilin gene specific 120bp amplified fragments were found in all specimens. These results suggested that intrinsic MDR1 gene expression is present in both the bladder tumor and normal bladder tissues, and implied that to a variety of chemotheraputic drugs in bladder tumors might be related to the expression of MDR1 gene. In addition, our data showed that RT-PCR is both sensitive and reliable for detecting MDR 1 gene expression.