ABC-ELISA检测血清曲霉多糖抗原

Detection of Aspergillus Carbohydrate Antigen with ABC-ELISA

将 ＡＢＣ－ＥＬＩＳＡ用于实验性烟曲霉感染兔血清中烟曲霉多糖抗原的检测，灵敏度可达 ２． ４ｕｇ／Ｌ。用本方法检测２０份经组织学证实的侵袭性烟曲霉感染兔血清，全部阳性，而３０份健康兔血清、１０份白念珠菌败血症血清，用本方法检测，仅２例阳性。根据组内变异系数（６．７％）和组间变异系数（８．６％）判断本方法具有较好的稳定性。

ABC-ELISA was established to detect aspergillus carbohydrate (AC) antigen for the diagnosis of aspergillosis. The assay was able to detect AC antigen as low as 2. 4ng/ml. 20 sera from experimentally infected rabbits with histologically proven invasive aspergillosis showed positive results,only 2 serum demonstrated to be positive among 30 healthy rabbits and 10 sera from rabbits with systemic candidiasis. The coeffcient variations of in the group (6. 7%) and between groups (8. 6%), showed that the assay has a good reproducibility.