神经干细胞的分离培养和鉴定

Isolation culture and identification of neural stem cells

目的研究神经干细胞分离、培养和分化鉴定技术。方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞,用无血清培养技术在体外进行培养、扩增、传代和诱导分化。采用免疫荧光技术,鉴定神经干细胞和分化的神经细胞。结果从胚胎大鼠的大脑皮质、海马、纹状体等组织获得了大量神经干细胞,可自我增殖并分化成为神经元、星形胶质细胞及少突胶质细胞。结论用上述方法分离培养的神经干细胞具有自我更新和增殖能力,并具有多向分化潜能。

[Objective] To investigate the methods of isolation, culature, differentiation and identification of the neural stem cells (NSCs). [Method] The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum. Immunofluorescence was applied to identify the neural stem cells and their potential of differentiation. [Result] Possess the ability of proliferation, self-reproduction and give rise neurons, astrocytes and oligodendrocytes. [Conclusion] The methods to isolate and culture neural stem cells from embryonic rat cortex, hippocampus and striatum is established. The cultured neural stem cells possess the ability of proliferation, self-reproduction and multi-differential potential.